Journal: Acta Pharmaceutica Sinica. B

Article Title: Neutralizing dentin sialophosphoprotein facilitates tumor vascular normalization in colorectal cancer by blocking the crosstalk between tumor cells and endothelial cells

doi: 10.1016/j.apsb.2026.06.011

Figure Lengend Snippet: Interleukin (IL)-17F upregulates DSPP expression and induces vascular abnormalities in CRC. (A) Western blot assays show the DSPP expression in HCT8 cells stimulated with a series of relevant inflammatory factors. (B, C) Real-time qPCR, Western blot assays show the effect of IL-17F and IL-17A on the DSPP expression in HCT8 cells. (D) The quantification of capillary tubule nodes, junctions, and branching length was performed on HUVECs exposed to CM from HCT8 cells treated with DSPP antibody ( α -DSPP), Cyclo(-RGDfK), and AZD6244. Data are presented as mean ± SD ( n = 3). (E) Western blot analysis demonstrates the impact of the interleukin-17 receptor A/interleukin-17 receptor C antibody on DSPP expression induced by IL-17F in HCT8 cells. (F) Chromatin immunoprecipitation assays verified the location of the p65 binding site within the promoter region of DSPP . (G) Luciferase reporter assay demonstrates the direct interaction of p65 with the promoter region of DSPP . (H) IF staining shows an increased phosphorylation of intracellular p65 in IL-17F-stimulated HCT8 cells. Scale bars = 10 μm. (I) Luciferase reporter assay demonstrates IL-17F affects the interaction of p65 to the DSPP promoter. (J, K) Western blot assay shows the nuclear factor kappa-B (NF- κ B) signaling pathway components expression. (L) In vivo bioluminescent images of orthotopic tumors in mice transplanted with the indicated HCT116 cells. (M) HE staining shows the metastasis of the orthotopic tumor in the liver and spleen. The quantification of the microvascular density is shown on the right. Scale bars = 100 μm. Data are presented as mean ± SD ( n = 4). (N) Representative images of IF staining of orthotopic tumors, and the quantification of Hypoxyprobe + areas, dextran leakage, and lectin perfusion of orthotopic tumor vessels. Scale bar = 20 μm. Data are presented as mean ± SD ( n = 5). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; ns, no significance.

Article Snippet: Inhibitors, including the mitogen-activated protein kinase (MAPK) kinase inhibitor selumetinib (AZD6244, MCE, Monmouth Junction, NJ, USA) or cyclo (-RGDfK) TFA (HY-P0023A, MCE), were introduced into the cultured cells.

Techniques: Expressing, Western Blot, Chromatin Immunoprecipitation, Binding Assay, Luciferase, Reporter Assay, Staining, Phospho-proteomics, In Vivo

Journal: ACS Omega

Article Title: Ultrasound-Induced Cavitation of Immunoliposomes for Rapid Drug Release, Deep Thrombus Penetration, and Enhanced Thrombolysis

doi: 10.1021/acsomega.5c13385

Figure Lengend Snippet: Schematic diagram of NH 4 HCO 3 decomposition, and pharmacodynamic mechanism of Lip-PEG-cRGD.

Article Snippet: RhoB 6B was purchased from Tokyo Chemical Industry Co., Ltd. Cyclo-RGDfK (cRGD) was obtained from Topscience Biotechnology Co., Ltd. (Shanghai, China).

Techniques:

Journal: ACS Omega

Article Title: Ultrasound-Induced Cavitation of Immunoliposomes for Rapid Drug Release, Deep Thrombus Penetration, and Enhanced Thrombolysis

doi: 10.1021/acsomega.5c13385

Figure Lengend Snippet: (A) Schematic illustration of the synthesis of DSPE-PEG 2000 -cRGD, together with the FT-IR spectra of DSPE-PEG 2000 -NH 2 , cRGD, and DSPE-PEG 2000 -cRGD and 1 H NMR spectra of DSPE-PEG 2000 -cRGD. (B) Size distribution of Lip, Lip-PEG, and Lip-PEG-cRGD. TEM image of Lip-PEG-cRGD.

Article Snippet: RhoB 6B was purchased from Tokyo Chemical Industry Co., Ltd. Cyclo-RGDfK (cRGD) was obtained from Topscience Biotechnology Co., Ltd. (Shanghai, China).

Techniques:

Journal: ACS Omega

Article Title: Ultrasound-Induced Cavitation of Immunoliposomes for Rapid Drug Release, Deep Thrombus Penetration, and Enhanced Thrombolysis

doi: 10.1021/acsomega.5c13385

Figure Lengend Snippet: (A) Fluorescence microscopy images of resting and activated platelets incubated with Lip, Lip-PEG, and Lip-PEG-cRGD. (B) Flow cytometry analysis of fresh platelets and thrombin-treated platelets incubated with FITC-loaded Lip, Lip-PEG, and Lip-PEG-cRGD. (C) Molecular docking models of cRGDfK with GPIIb/IIIa in inactive and activated states.

Article Snippet: RhoB 6B was purchased from Tokyo Chemical Industry Co., Ltd. Cyclo-RGDfK (cRGD) was obtained from Topscience Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Fluorescence, Microscopy, Incubation, Flow Cytometry

Journal: ACS Omega

Article Title: Ultrasound-Induced Cavitation of Immunoliposomes for Rapid Drug Release, Deep Thrombus Penetration, and Enhanced Thrombolysis

doi: 10.1021/acsomega.5c13385

Figure Lengend Snippet: Thrombolytic efficacy of UK@Lip-PEG-cRGD in a mouse carotid artery thrombosis model and evaluation of bleeding risk using a tail bleeding assay. (A) Schematic illustration of the carotid artery thrombolysis experiment in mice. (B) Representative images of continuous vascular sections from uniform carotid artery tissue excised from mice in different treatment groups: PBS control, thrombosis model, free UK (100 U/g), nonultrasound (free UK, 100 U/g), and ultrasound (UK@Lip-PEG-cRGD, 100 U/g). Scale bar = 50 μm. (C) Quantification of the thrombolytic efficacy based on thrombus size, measured using ImageJ image analysis software. (D) Tail bleeding test in mice administered PBS, free UK (100 U/g), UK@Lip-PEG-cRGD (100 U/g), and UK@Lip-PEG-cRGD (100 U/g) with ultrasound. Four mice per group. Data are presented as mean ± standard error of the mean (SEM).

Article Snippet: RhoB 6B was purchased from Tokyo Chemical Industry Co., Ltd. Cyclo-RGDfK (cRGD) was obtained from Topscience Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Control, Software

Journal: Advanced Science

Article Title: Legumain Restrains Granuloma Formation by Inhibiting mTORC1/STAT1‐Mediated M1 Macrophage Polarization in Sarcoidosis

doi: 10.1002/advs.202520635

Figure Lengend Snippet: LGMN–integrin αvβ3 interaction suppresses STAT1‐mediated M1 polarization. (a) Co‐immunoprecipitation of ITGAV and LGMN in the lung homogenate from the P. acnes ‐induced mouse model. (b) Co‐immunoprecipitation of ITGB3 and LGMN in the lung homogenate from the P. acnes ‐induced mouse model. (c) Western blot analysis of iNOS expression in BMDMs after stimulation with LPS and IFN‐γ with or without integrin αvβ3 antagonist Cyclo(‐RGDfK). (d) Western blot analysis of the levels of p‐STAT1 (Ser727 and Tyr701) and STAT1 in vector‐ or Lgmn plasmid‐treated RAW264.7 cells stimulated with LPS and IFN‐γ. (e) Western blot analysis of p‐STAT1 (Ser727 and Tyr701) expression in the lungs from WT and Lgmn ‐KO mice after P. acnes challenge (n = 5 each). (f) Results for co‐immunostaining of LGMN (green), p‐STAT1 (Ser727) (yellow), and Mac‐2 (red) in the lung sections from WT and Lgmn‐ KO mice after P. acnes challenge (n = 5 each). The nuclei were stained blue by DAPI. Representative images were captured at ×400 magnification. Bar = 25 µm. The data are represented as the mean ± SEM. An unpaired t ‐test (f) and ordinary one‐way ANOVA (c, d, e) were applied. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: For M2 polarization, BMDMs were treated with 20 ng/mL recombinant mouse IL‐4 (PeproTech, Cranbury, NJ, USA) and 20 ng/mL recombinant mouse IL‐13 (PeproTech, Cranbury, NJ, USA) for 24 or 48 h. For the indicated experiments, 10 μM Cyclo(‐RGDfK) (MedChemExpress, Shanghai, China), 1 μM Rapa (MedChemExpress, Shanghai, China), or 50 μM F‐ara‐A (MedChemExpress, Shanghai, China) was added during M1 polarization.

Techniques: Immunoprecipitation, Western Blot, Expressing, Plasmid Preparation, Immunostaining, Staining